Vitamin B12 protocol

Standard

Deficiency loading

25u

1,000 mcg · Daily ×7 then weekly

Target serum B12 400–900 pg/mL.

Maintenance

25u

1,000 mcg · Monthly

Once repleted.

MIC protocol

25u

1 mg/mL in blend · M/W/F

As component of lipotropic blend.

Phase	How often	Dose	Units	Notes
Deficiency loading	Daily ×7 then weekly	1,000 mcg	25u	Target serum B12 400–900 pg/mL.
Maintenance	Monthly	1,000 mcg	25u	Once repleted.
MIC protocol	M/W/F	1 mg/mL in blend	25u	As component of lipotropic blend.

Loading: 1000 mcg daily x 7 days then weekly x 4 weeks for deficiency.

Cycle & timing

Cycle: Monthly maintenance ongoing. Loading: 1000 mcg daily x 7 days then weekly x 4 weeks for deficiency. No cycling required for maintenance.

Timing: Weekly IM (maintenance); daily for repletion phase.

Where: SubQ or IM (IM preferred for deficiency repletion).

What to expect

Energy improvement within days of loading in deficient individuals. Neurological symptoms may improve over 4–8 weeks. Target serum B12: 400–900 pg/mL.

How much to buy

For 4-wk @ 1000 mcg × 7/wk:

3

× 10mg vials

Men & women

Unisex, same dosing for anyone; no cycle effect. Safe and commonly used in pregnancy at normal doses.

Dilution calculator

Set to the guide's typical mix for Vitamin B12. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

25u

Volume

0.25 mL

Strength

4 mg/mL

Doses per vial

10

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
10 mg	2.5 mL	4 mg/mL

10 mg: 1 unit = 0.04 mg

Storage

MixedSolution: room temp or refrigerated. Use within 30 days. Protect from light.

Don't use if: Polycythemia vera · Cobalt allergy · Leber’s optic neuropathy (cyanocobalamin form specifically)

Side effects

Rare. Mild injection-site irritation.

Drug interactions

Proton pump inhibitors (omeprazole, pantoprazole, esomeprazole) and H2 blockers: long-term use reduces oral B12 absorption; injectable B12 bypasses this and is the preferred route in PPI users. Metformin: chronic use depletes B12; check B12 + MMA annually in metformin users. Neomycin and other oral aminoglycosides: reduce intestinal B12 absorption; injectable route bypasses. Colchicine, chloramphenicol: interfere with B12 absorption or response to B12 therapy. Nitrous oxide: inactivates B12; avoid recreational or repeat anesthetic exposure during deficiency treatment. Folate supplementation: high-dose folate can mask B12 deficiency on CBC while neurological deterioration continues, always check both before high-dose folate.

Labs

Compound-specific: CBC (baseline – confirm macrocytic anemia resolution), Serum B12 (baseline + Week 8; target 400–900 pg/mL); MMA + homocysteine if neurological symptoms. Out of range? Low B12 = deficiency (fatigue, tingling, brain fog) → keep supplementing until it's mid-range.

What these labs mean →

Pregnancy & breastfeeding

Safe and commonly used in pregnancy at normal doses.

Warnings

Methylcobalamin preferred over cyanocobalamin for most uses.

Patient education

Cyanocobalamin contraindicated in Leber’s; use methylcobalamin or hydroxocobalamin.

These apply on top of the universal safety rules.

Pairs well with

Lipo-C

lipotropic stack

LC120

NAD+

cellular energy

NAD+

GLP-1 agonists

counters B12 depletion

Avoid with

Polycythaemia vera

B12 amplifies erythrocyte production

Cobalt allergy

Can Vitamin B12 + LC120 share a syringe?

Give it its own pin. The guide says this one goes by itself.

What it does

Essential B-vitamin for nerve function, red blood cell formation, and energy metabolism. Cofactor for methionine synthase (methylation) and methylmalonyl-CoA mutase (energy metabolism).

References

researchdosing peptidedosages DailyMed PubMed ClinicalTrials Drugs.com

From the guide, page 106.