Melatonin protocol

Standard

Nightly

25–150u

0.5–3 mg · Pre-bed

SubQ 30–60 min before bed. Start low, more is not better for sleep.

Phase	How often	Dose	Units	Notes
Nightly	Pre-bed	0.5–3 mg	25–150u	SubQ 30–60 min before bed. Start low, more is not better for sleep.

Units at 2 mg/mL. Low doses (0.5–1 mg) often work better and cause less morning grogginess than high doses.

Cycle & timing

Cycle: As needed; can be used nightly or for jet-lag/shift-work resets.

Timing: 30–60 min before desired sleep; for jet lag, time to the destination night.

Where: SubQ, abdomen. (Oral/sublingual is the more common route.)

What to expect

Faster sleep onset and circadian shifting. Not a sedative, it signals timing, so a dark, low-stimulation environment matters.

How much to buy

For weeks of nightly low-dose use:

1

× 10mg vials

Men & women

Unisex, dose by protocol, not sex; start low. Discuss with a prescriber in pregnancy / breastfeeding.

Dilution calculator

Set to the guide's typical mix for Melatonin. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

50u

Volume

0.50 mL

Strength

2 mg/mL

Doses per vial

10

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
10 mg	5 mL	2 mg/mL

10 mg: 1 unit = 0.02 mg. A 1 mg dose = 50 units.

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 4 weeks. Protect from light.

Don't use if: Pregnancy and lactation · Active autoimmune disease (high doses are immune-stimulating) · Hypersensitivity

Stop if you notice

Persistent daytime grogginess or worsened sleep
Mood changes
Pregnancy

Side effects

Morning grogginess (esp. high doses), vivid dreams, mild next-day drowsiness, headache. Generally very safe.

Drug interactions

Sedatives / alcohol: additive drowsiness. Anticoagulants: possible additive effect, monitor. Antihypertensives / antidiabetics: mild additive lowering. Fluvoxamine and some CYP1A2 inhibitors raise melatonin levels.

Labs

None routinely required

What these labs mean →

Warnings

Next-day drowsiness, don't drive until you know your response. High doses can worsen sleep quality and cause grogginess. May lower blood pressure and blood glucose slightly. Can interact with seizure threshold in susceptible individuals.

Patient education

It signals "night," it doesn't knock you out; pair with darkness and a wind-down. Low dose first; high doses often backfire. Don't drive after dosing until you know your response. Most people use oral; injectable is optional.

These apply on top of the universal safety rules.

Pairs well with

DSIP

deep sleep

DSIP

Epithalon

pineal/longevity

Epithalon

Selank

evening anxiety

Selank

Avoid with

Daytime dosing

disrupts rhythm

Autoimmune disease

immune-stimulating at high doses

Pregnancy

Operating vehicles after dosing

Can Melatonin + DSIP share a syringe?

Give it its own pin. The guide says this one goes by itself.

What it does

Melatonin is the pineal hormone that regulates the sleep-wake cycle and acts as a potent antioxidant. It shifts circadian timing (useful for jet lag/shift work) and promotes sleep onset. Oral is the usual route; injectable delivers a faster, more predictable dose. Lower doses (0.5–1 mg) are often better for sleep than high doses.

References

researchdosing peptidedosages PubMed thepeptidereport

From the guide, page 65.