Kisspeptin-54 protocol

Standard

IVF trigger

6.4 nmol/kg · Single dose

Clinical IVF protocol. Oocyte maturation trigger vs hCG.

HPG restoration

2–6u

100–300 mcg · 3× weekly

Community HPG axis stimulation. Lower desensitisation risk than K-10 due to longer natural pulse.

Off

Cycle-dependent

No standard off period, use pulsatile protocol only.

Phase	How often	Dose	Units	Notes
IVF trigger	Single dose	6.4 nmol/kg	–	Clinical IVF protocol. Oocyte maturation trigger vs hCG.
HPG restoration	3× weekly	100–300 mcg	2–6u	Community HPG axis stimulation. Lower desensitisation risk than K-10 due to longer natural pulse.
Off	Cycle-dependent	–	–	No standard off period, use pulsatile protocol only.

Cycle & timing

Cycle: Pulsatile 3× weekly only. Daily dosing causes GPR54 desensitisation.

Timing: IVF trigger single shot or weekly.

Where: SubQ abdomen. IV for IVF protocols (clinical setting only).

What to expect

LH surge within 30–60 min of injection. Testosterone rise within 2–4 hours. Sustained HPG activation over 3× weekly protocol builds over 4–6 weeks. Used as IVF trigger: oocyte maturation within 36–38 hrs at clinical dose.

How much to buy

For 4-wk @ 300 mcg × 3/wk:

1

× 5mg vials

Men & women

Men: Raises LH and testosterone, investigational for low-T and low libido.

Women: The LH/FSH response is hugely cycle-dependent, far stronger just before ovulation. Timing is everything.

Dilution calculator

Set to the guide's typical mix for Kisspeptin-54. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

6u

Volume

0.06 mL

Strength

5 mg/mL

Doses per vial

16

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
5 mg	1 mL	5 mg/mL

5 mg: 1 unit = 0.05 mg

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, use within 28 days.

Don't use if: Hormone-sensitive malignancy (prostate cancer outside intentional ADT, breast cancer, ovarian cancer) · Active thromboembolic disease · Untreated thrombophilia · Pregnancy (except specific fertility indications under specialist supervision) · Polycythemia vera

Stop if you notice

Hematocrit >54%, donate blood or hold therapy
Estradiol persistently above target despite AI
OHSS symptoms (abdominal distension, weight gain, dyspnea)
Thrombosis symptoms (calf swelling, chest pain, dyspnea, neuro)
New breast tenderness or growth (gynecomastia workup)

Side effects

Facial flushing, warmth, mild abdominal discomfort (most common). Transient dizziness with IV administration. Generally very well tolerated.

Drug interactions

AIs (anastrozole, letrozole): co-administered to manage E2; over-suppression worsens lipids and bone; SERMs (clomiphene, tamoxifen): redundant overlap via central HPG drive; Exogenous testosterone: shuts down LH/FSH, HCG efficacy reduced unless TRT paused or microdosed; Anticoagulants (warfarin, DOACs): elevated E2 raises thromboembolic risk; coordinate if chronic; Insulin / antidiabetics: HCG may shift glucose at high doses, monitor diabetic patients

Labs

Total & free testosterone, LH, FSH, sensitive E2; baseline, mid-cycle, end (LH/FSH Day 0/10/30); SHBG and prolactin at baseline; CBC + hematocrit (polycythemia surveillance); Lipid panel; PSA in men >40 at baseline; Semen analysis baseline + quarterly for fertility protocols; D-dimer if clotting symptoms; Out of range? Low testosterone or high estradiol = the hormone axis needs tuning; hematocrit >54% = blood too thick (donate or hold).

What these labs mean →

Warnings

Estradiol elevation common; aromatase inhibitor may be needed; Thromboembolic risk with elevated estradiol; Ovarian hyperstimulation syndrome (OHSS) in females on HCG: can be life-threatening; Mood changes, gynecomastia in males; HCG raises both T and E2; estradiol monitoring is mandatory; Longer half-life than K-10; lower desensitization risk but same axis effects

Patient education

Watch for clotting symptoms: leg swelling, calf pain, chest pain, sudden shortness of breath; Estrogen side effects (gynecomastia, mood) manageable with AI under physician guidance; Fertility protocols: 70+ days before semen analysis changes

These apply on top of the universal safety rules.

Pairs well with

HCG

additive HPG stimulation

HCG

CJC-1295 No DAC + Ipamorelin

anabolic environment

CJC-1295 (No DAC) + Ipamorelin

BPC-157 / TB-500

recovery during cycle

BPC-157 TB-500 (Thymosin Beta-4)

PT-141

sexual response synergy

PT-141 (Bremelanotide)

Avoid with

Used too frequently

GPR54 desensitisation

Hormone-sensitive cancers

Pregnancy

Can Kisspeptin-54 + HCG share a syringe?

Depends on timing. Reproductive and hormonal peptides are compatible, but doses and timing vary widely. Combine only what you truly co-time. If unsure, separate.

What it does

Full-length 54-amino acid kisspeptin, the endogenous master HPG axis regulator. Binds KISS1R/GPR54 on GnRH neurons in hypothalamic arcuate nucleus and AVPV to trigger pulsatile GnRH release → LH/FSH → testosterone.

Approved drug

Approved fertility options: clomiphene, hCG, injectable gonadotropins (FSH/hMG).

References

peptidedosages PubMed thepeptidereport Abbara 2015

From the guide, page 49.