BR226 protocol · PepGuide

Standard

Pre-bed

100u

1 mL · Nightly

Inject 30–60 min before sleep. Full vial = 10 nights.

Off

1–2 weeks

Optional break after 8 weeks continuous to avoid melatonin receptor downregulation.

Phase	How often	Dose	Units	Notes
Pre-bed	Nightly	1 mL	100u	Inject 30–60 min before sleep. Full vial = 10 nights.
Off	1–2 weeks	–	–	Optional break after 8 weeks continuous to avoid melatonin receptor downregulation.

Cycle & timing

Cycle: 4–8 weeks on / 1–2 weeks off, or as needed. Avoid daily continuous use beyond 8 weeks (melatonin receptor downregulation).

Timing: Daily SubQ, anytime; see notes for compound-specific timing.

Where: SubQ abdomen or outer thigh.

What to expect

Drowsiness within 20–30 min. Deeper subjective sleep over 1–2 weeks of consistent pre-bed use.

Men & women

Follows its components, same protocol for men and women; no meaningful cycle effect. Avoid in pregnancy.

Recommended mixes

Vial	Water	Strength
10 ml	Solution, no reconstitution needed	GABA 10 mg/mL; Melatonin 0.1 mg/mL; Arginine 100 mg/mL; Glutamine 2.5 mg/mL

10 ml: 1 unit = 0.10 mg

Storage

MixedSolution: refrigerate 36–46°F. Use within 28 days. Protect from light (melatonin photodegrades).

Side effects

Vivid dreams (melatonin), AM grogginess if dosed too close to wake. Rare flushing (arginine vasodilation).

Labs

Compound-specific: CBC + CMP baseline; no compound-specific markers routinely required

What these labs mean →

Patient education

Aseptic technique: alcohol swab the vial septum and the injection site, use a single-use sterile needle for every injection, never share vials or needles between people. Sharps disposal in an FDA-cleared sharps container, never household trash. Cold chain: if a refrigerated peptide reaches room temperature for more than the labeled stability window, discard it. Mark the date of first puncture on every multi-dose vial. Inspect every vial before injection: clear solution (unless labeled otherwise), no particulates, no cloudiness, no color change. Rotate injection sites to avoid lipoatrophy, induration, and infection. Carry a record of what you’re injecting if you need emergency care.

These apply on top of the universal safety rules.